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SARS-CoV-2 Nucleic Acid Test Kit (PCR-Fluorescence Probe Method)
SARS-CoV-2 Nucleic Acid Test Kit (PCR-Fluorescence Probe Method)

SARS-CoV-2 Nucleic Acid Test Kit (PCR-Fluorescence Probe Method)

The ORF1ab and N gene sequences of SARS-CoV-2 are used as target sequences and the human RNaseP gene is used as internal control. For this test kit, a specific primer and a fluorescence probe are designed. Reverse transcriptase, RNA enzyme inhibitor, DNA polymerase and four deoxynucleotide monomers are formulated. By employing the polymerase chain reaction (PCR) and fluorescence probe techniques, the one-step method is used to rapidly qualitatively test if the corresponding ORF1ab and N genes exist in the clinical sample. 


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SARS-CoV-2 Nucleic Acid Test Kit (PCR-Fluorescence Probe Method) Details

Product Description

SARS-CoV-2 Nucleic Acid Test Kit is a real-time PCR test intended for the qualitative detection in ORF1ab and N genes of SARS-CoV-2 viral nucleic acids in pharyngeal specimens (oropharyngeal swabs, nasopharyngeal swabs) from individuals who are suspected of COVID-19 by their healthcare provider. 


SARS CoV 2 Nucleic Acid Test Kit


Work Flow

Step 1 Nucleic Acid Extraction 


PCR Fluorescence Probe Method


Step 2 RT-PCR Amplification and Analysis 


SARS CoV 2 Nucleic Acid

Standard Configurations

Key Components

Ordering Information

Number of Test / Kit24 tests
Sample TypeOropharyngeal swabs, Nasopharyngeal swabs
Acceptable Real-time PCR SystemLEPU Lepgen-96, Roche LightCycler 480 series Bio-Rad CFX96
Reagent Preparation Condition4 weeks at 2℃ ~8℃
Reagent Storage Condition6 months at -25℃ ~ -15℃, The freeze/thaw cycles should not exceed five times


Component

Qty.

Volume

Ingredients

Cov-PCR Reaction Liquid

1

460μl/vial

ORF1ab and N genes primer

and probe, Internal control

RNase P gene primer and

probe, Deoxyribonucleoside

triphosphate

Cov-enzyme

1

24μl/vial

DNA polymerase, Reverse

transcriptase, Recombinant

RNase inhibitor.

Cov-Negative Control

1

420μl/vial

DEPC treated water

Cov-Positive Control

1

420μl/vial

ORF1ab and N genes, RNase P

gene armored RNA solution


Catalogue No.SARS-CoV-2 Nucleic Acid Test Kit
(PCR-Fluorescence Probe Method)


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