The ORF1ab and N gene sequences of SARS-CoV-2 are used as target sequences and the human RNaseP gene is used as internal control. For this test kit, a specific primer and a fluorescence probe are designed. Reverse transcriptase, RNA enzyme inhibitor, DNA polymerase and four deoxynucleotide monomers are formulated. By employing the polymerase chain reaction (PCR) and fluorescence probe techniques, the one-step method is used to rapidly qualitatively test if the corresponding ORF1ab and N genes exist in the clinical sample.
SARS-CoV-2 Nucleic Acid Test Kit is a real-time PCR test intended for the qualitative detection in ORF1ab and N genes of SARS-CoV-2 viral nucleic acids in pharyngeal specimens (oropharyngeal swabs, nasopharyngeal swabs) from individuals who are suspected of COVID-19 by their healthcare provider.
Step 1 Nucleic Acid Extraction
Step 2 RT-PCR Amplification and Analysis
|Number of Test / Kit||24 tests|
|Sample Type||Oropharyngeal swabs, Nasopharyngeal swabs|
|Acceptable Real-time PCR System||LEPU Lepgen-96, Roche LightCycler 480 series Bio-Rad CFX96|
|Reagent Preparation Condition||4 weeks at 2℃ ~8℃|
|Reagent Storage Condition||6 months at -25℃ ~ -15℃, The freeze/thaw cycles should not exceed five times|
Cov-PCR Reaction Liquid
ORF1ab and N genes primer
and probe, Internal control
RNase P gene primer and
DNA polymerase, Reverse
DEPC treated water
ORF1ab and N genes, RNase P
gene armored RNA solution
|Catalogue No.||SARS-CoV-2 Nucleic Acid Test Kit|
|(PCR-Fluorescence Probe Method)|
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