The detection principle of the new coronavirus (2019-nCoV) IgM antibody detection kit (colloidal gold method) is to label mouse anti-human IgM with colloidal gold, and at the same time, the new coronavirus antigen is coated on the nitrocellulose membrane, and the colloidal gold immunochromatography is used to indirectly The principle of the method achieves the purpose of detecting the novel coronavirus IgM antibody in human serum.
The principle of the new coronavirus IgM antibody detection kit (fluorescence immunochromatography) is based on dry fluorescent labeling technology and immunochromatography technology. Whole blood, serum or plasma are used as samples, and it is equipped with an automatic fluorescence immunoassay quantitative analyzer. Automatic puncture and sample injection to achieve "sample in, result out", avoiding the generation of aerosols to the greatest extent, and reducing the risk of pollution to operators and the environment.
The principle of the new coronavirus IgM antibody detection kit (enzyme-linked immunosorbent assay) is to apply the capture enzyme-linked immunosorbent assay method, coat the reaction plate with anti-human IgM monoclonal antibody, add calibrators or diluted samples, The viral IgM antibody combines with the coated monoclonal antibody to form an anti-human IgM antibody-IgM antibody complex. After washing, the HRP-labeled 2019-nCoV N protein antigen (E protein antigen or S protein antigen) is added to form an anti-human IgM antibody. -IgM antibody-2019-nCoV N protein antigen-HRP complex.
After washing the plate, the TMB substrate was added, and the HRP catalyzed substrate on the complex formed a blue substance, which turned yellow after adding the stop solution, and had a specific absorption peak at 450nm wavelength. Qualitative detection of 2019-nCoV IgM antibodies in human serum by peak level.
The principle of the new coronavirus antibody detection kit (chemiluminescence method) is that the recombinant protein of the virus is labeled with high-tech material magnetic beads, which is used to bind the virus antibody IgM in the blood sample, and then use the secondary antibody to identify the antibody.
The secondary antibody is coupled with alkaline phosphatase, and a light signal is generated after adding the substrate. The chemiluminescence instrument detects photons through a high-sensitivity PMT (photomultiplier tube), thereby achieving high-sensitivity detection at the pg (picogram) level.